The ATP-binding cassette (ABC) transporter Bpt1p mediates vacuolar sequestration of glutathione conjugates in yeast

Autor(en)

Markus Klein, Yasmine M. Mamnun, Thomas Eggmann, Christoph Schüller, Enrico Martinoia, Karl Kuchler

Abstrakt

Vacuolar sequestration or cellular extrusion of glutathione-conjugated xenobiotics and catabolites by ATP-binding cassette (ABC) transporters is an important detoxification mechanism operating in many species. In this study, we show that the yeast ABC transporter Bpt1p, a paralogue of Ycf1p, acts as an ATP-dependent vacuolar pump for glutathione conjugates. Bpt1p, which is inhibited by vanadate and glibenclamide, accounts for one third of the total vacuolar transport of glutathione conjugates. Furthermore, immunoblot analyses show that Bpt1p levels are strongly elevated in early stationary phase, consistent with a function of Bpt1p in vacuolar detoxification. Π2002 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.

Organisation(en)

Institut für Kultur- und Sozialanthropologie, Department für Biochemie und Zellbiologie, Department für Mikrobiologie, Immunbiologie und Genetik

Externe Organisation(en)

Universität Zürich (UZH), Vienna BioCenter, Université de Neuchâtel

Journal

FEBS Letters

Band

520

Seiten

63-67

Anzahl der Seiten

5

ISSN

0014-5793

Publikationsdatum

2002

Peer-reviewed

Ja

ÖFOS 2012

1060 Biologie

Link zum Portal

https://ucrisportal.univie.ac.at/de/publications/the-atpbinding-cassette-abc-transporter-bpt1p-mediates-vacuolar-sequestration-of-glutathione-conjugates-in-yeast(79cbc5f9-3c70-4291-89fb-4367d5bb2b25).html